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T)) represents the descriptor of the analyte retention factor at the column inlet as a function of time. 91) where ϕ 0 denotes the mobile phase composition at the start of the gradient (ϕ ϭ ϕ0 ϭ ϕЈt). The method has been proposed for the prediction of retention data in isocratic systems from data measured in gradient elution and vice versa [84]. Similar calculation methods may be very important in the analysis of natural extracts containing pigments with highly different chemical structure and retention characteristics.

The information content of a fingerprint (Φ ) can be calculated after normalizing with its overall peak area equal to one. Each real chromatographic response is divided by the sum of each real chromatographic response. The information content is calculated by the summation of the ratios mentioned above. The advantages of this calculation method are that each characteristic of the chromatogram is taken into consideration, the peaks need not to be identified, and the noise exerts a negligible influence on the calculation.

They can facilitate the determination of the authenticity and traceability of the natural product under investigation. Chromatographic fingerprints are chromatographic patterns of complicated multicomponent samples without the exact identification of each sample component. The application of hyphenated techniques highly increases their information content. The practical value of a chromatographic fingerprint increases with increasing separation capacity of the RP-HPLC system and the concentration distribution of each absolute molecule.

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